How it works

Confocal fluorescence microscopy is the gold standard for imaging 3D samples such as organoids, spheroids or large tissue samples. However, the thickness of these samples leads to light absorption and scattering. This makes it hard to inspect images visually as we cannot differentiate between a lack of marker (e.g. a necrotic core) or light loss.

Our physics-based innovation measures the amount of absorption and scattering of both excitation and emission light. Using these measurements we can correct for absorption and scattering in the sample to obtain a measure of the fluorophore concentration that is unaffected by the sample thickness, optical properties, or which plane is being imaged.

Our solution allows, for the first time using confocal microscopy, measuring spatial differences in fluorophore concentration within and between organoids. Using this, we understand whether a lack of signal in the center of an organoid is due to a lack of fluorescent marker or simply an artifact from light not reaching the center of the organoid.

We present our software: Jellyfish

Confocal microscopy at its full potential

Get a measure of fluorophore constration from your confocal microscopy images!
Contact us at contact@ciprocity.bio to discuss your project,
and the challenges you encounter when imaging your 3D samples.

Who we are

A team of enthousiastic researchers striving for better science

Our adventure started two years ago when trying to adress a recurring problem in our research projects using spheroid imaging: the gold standard for 3D sample imaging wasn't allowing for quantitivative marker analysis.
We are now aiming to improve reliability of microscopy by releasing a tool to analyze confocal images.

Petter Säterskog
PhD in Physics
Ciprocity CEO

Loay Mahmoud
PhD student,
NTNU/KI

Louise Gsell
PhD student,
KI/Tokyo University

Antony Cougnoux
PhD in Microbiology,
The Hide & Seek Foundation